Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 37 21 59.73 47.62 4.00 2.00 CAGAGACCTGTGGAAAAATGC
RIGHT PRIMER 313 20 58.70 55.00 7.00 0.00 CGTGTGACACTGTTGGTGAG
SEQUENCE SIZE: 474
INCLUDED REGION SIZE: 474
PRODUCT SIZE: 277, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
1 GTAGTTACATGGCTCTATCTATTTCTCAAAACTCAACAGAGACCTGTGGAAAAATGCAGT
>>>>>>>>>>>>>>>>>>>>>
61 TCTCAATATGACAACCACACAATCTTAAACCAGTTAATATTAAAGTTGTGTGGAGACAGC
121 AGTGATGCCACCATCTATAAGTTGGAAGTAAGTAAACTAAAGCTAAGAATACCTAAGAGA
181 TCTNTTCTAAACCATTCAGGCTGTAGTGATACAATCAGGACATCACTCCAGGTCACCAAA
241 CTCTTCCACAACACTACATCAGAAATTAATATAAGTACTTTTAAAGAATGTCACTCACCA
<<<<<<<
301 ACAGTGTCACACGGGACAACCCAACATTTTACTGTGTTATCAGAAGAAGTCAGACATATG
<<<<<<<<<<<<<
361 CTAAGACTAATAAAGCGAATCAGATATTTAGTTTAACATAAGAAATAAAGTACNNNNNNN
421 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 37 21 59.73 47.62 4.00 2.00 CAGAGACCTGTGGAAAAATGC
RIGHT PRIMER 314 19 59.58 57.89 7.00 1.00 CCGTGTGACACTGTTGGTG
PRODUCT SIZE: 278, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER 39 21 59.73 47.62 4.00 2.00 GAGACCTGTGGAAAAATGCAG
RIGHT PRIMER 313 20 58.70 55.00 7.00 0.00 CGTGTGACACTGTTGGTGAG
PRODUCT SIZE: 275, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
3 LEFT PRIMER 39 21 59.73 47.62 4.00 2.00 GAGACCTGTGGAAAAATGCAG
RIGHT PRIMER 314 19 59.58 57.89 7.00 1.00 CCGTGTGACACTGTTGGTG
PRODUCT SIZE: 276, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER 37 21 59.73 47.62 4.00 2.00 CAGAGACCTGTGGAAAAATGC
RIGHT PRIMER 318 20 61.72 55.00 7.00 1.00 TGTCCCGTGTGACACTGTTG
PRODUCT SIZE: 282, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 1382 21 0 0 137 192 597 160 5 52 0 8 210
Right 1252 85 0 0 227 148 508 131 0 42 0 9 102
Pair Stats:
considered 13674, unacceptable product size 9741, high any compl 42, high end compl 586, ok 3305
primer3 release 0.9
(primer3_www_results.cgi v 0.1 beta 1a)