Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 75 20 59.96 50.00 3.00 2.00 CTGGCTTCCATTTCTCTTGC
RIGHT PRIMER 388 22 59.85 40.91 4.00 0.00 CCAGAATTGGGTTTTCTTGTTC
SEQUENCE SIZE: 627
INCLUDED REGION SIZE: 627
PRODUCT SIZE: 314, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00
1 ATAACTGTACACAGGACAAAGAGTTCCCTTTTAATAATCTGAGTATGGCATAAAAGATAG
61 TTAGGTCAGAAAACCTGGCTTCCATTTCTCTTGCACATTCTCCATAATACTTGTCTTTTA
>>>>>>>>>>>>>>>>>>>>
121 ACTGATTTGTGAGGCCAGTTATCATGGTATAAGGTGACCCTATAGGTATGAAAGTAGGAT
181 TATGTGCCAAGAATCTAATGACTTCTAACCATAGCTGGGAAAGCTCTGAAATTTAGTCCA
241 ACCAGCATGAAAATAACTCTTCATAATAAAATATATGTGTGAATGGGCTTTCTATCAGAT
301 CCTTATTCTATTAGGATTTGACAGGATATCTACTAAAGTAGACATTCTGAGTGCACTCAT
361 ATAAGAGAACAAGAAAACCCAATTCTGGAGACTAAAACTACAGAGCAATTAATCATGTTG
<<<<<<<<<<<<<<<<<<<<<<
421 TTATGAATAGTATATTAAGTAATATGCCATTATGATATATTTGACTTCTGACCTAACAAA
481 ACCATGTCACTTTGAAGATATCATATTTTAAATACAATGTTAAACAGTTGAATTTAAATT
541 TCATATTCTTTAAATATCCTTTGTCATCATTTTGGTTGCTTATTCTTTTTTCTTTCTAGA
601 CCTGCTGCTAAAATTATCTTTCAACTG
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 77 20 59.82 50.00 4.00 2.00 GGCTTCCATTTCTCTTGCAC
RIGHT PRIMER 388 22 59.85 40.91 4.00 0.00 CCAGAATTGGGTTTTCTTGTTC
PRODUCT SIZE: 312, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
2 LEFT PRIMER 74 20 59.81 50.00 3.00 0.00 CCTGGCTTCCATTTCTCTTG
RIGHT PRIMER 388 22 59.85 40.91 4.00 0.00 CCAGAATTGGGTTTTCTTGTTC
PRODUCT SIZE: 315, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER 75 20 59.96 50.00 3.00 2.00 CTGGCTTCCATTTCTCTTGC
RIGHT PRIMER 389 20 57.64 40.00 4.00 1.00 TCCAGAATTGGGTTTTCTTG
PRODUCT SIZE: 315, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER 75 20 59.96 50.00 3.00 2.00 CTGGCTTCCATTTCTCTTGC
RIGHT PRIMER 390 21 58.68 42.86 4.00 1.00 CTCCAGAATTGGGTTTTCTTG
PRODUCT SIZE: 316, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 2061 0 0 0 232 313 1060 94 0 10 0 15 337
Right 2168 0 0 0 587 300 945 57 0 6 7 14 252
Pair Stats:
considered 332, unacceptable product size 256, high end compl 4, ok 72
primer3 release 0.9
(primer3_www_results.cgi v 0.1 beta 1a)