Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 111 21 59.40 47.62 4.00 1.00 GGATTGAAGACCATCACAAGG
RIGHT PRIMER 492 20 59.55 55.00 1.00 0.00 TCCACACACACCCTTCTCAC
SEQUENCE SIZE: 541
INCLUDED REGION SIZE: 541
PRODUCT SIZE: 382, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
1 TATATTATAGGGTAACCAACTAATTTCTTACTGGGTTTGATGCCTACCTACTCCACAGAA
61 AGAAATCTATATCTGCTACTGTGAACTTCATCAATAGGCCCATGCCTATAGGATTGAAGA
>>>>>>>>>>
121 CCATCACAAGGAAACTATTGTTATTGTTTTATTAAGTAGATATGATGTGTCTATCAATCT
>>>>>>>>>>>
181 GCTTTCTAAATAACTGTATAAGTACTATATAACCATATAACTANNNNNNNNNNNNNNNNN
241 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTA
301 TATATATATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAGGGAAATGCTCAGAATT
361 AACTGAACAAGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
421 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTGAGAAG
<<<<<<<<
481 GGTGTGTGTGGAAATTGGCTTCCTAACAGGACACGGATGCTAAATTCTAGAACTCACAGC
<<<<<<<<<<<<
541 T
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 86 20 60.44 50.00 6.00 2.00 CTTCATCAATAGGCCCATGC
RIGHT PRIMER 492 20 59.55 55.00 1.00 0.00 TCCACACACACCCTTCTCAC
PRODUCT SIZE: 407, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
2 LEFT PRIMER 111 21 59.40 47.62 4.00 1.00 GGATTGAAGACCATCACAAGG
RIGHT PRIMER 503 20 60.36 50.00 4.00 0.00 GGAAGCCAATTTCCACACAC
PRODUCT SIZE: 393, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER 86 20 60.44 50.00 6.00 2.00 CTTCATCAATAGGCCCATGC
RIGHT PRIMER 503 20 60.36 50.00 4.00 0.00 GGAAGCCAATTTCCACACAC
PRODUCT SIZE: 418, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
4 LEFT PRIMER 111 21 59.40 47.62 4.00 1.00 GGATTGAAGACCATCACAAGG
RIGHT PRIMER 500 20 60.82 50.00 4.00 0.00 AGCCAATTTCCACACACACC
PRODUCT SIZE: 390, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 1299 252 0 0 143 159 451 116 8 24 0 6 140
Right 1365 254 0 0 149 148 491 127 0 22 0 10 164
Pair Stats:
considered 4886, unacceptable product size 2422, high end compl 164, ok 2300
primer3 release 0.9
(primer3_www_results.cgi v 0.1 beta 1a)