Primer3 Output



No mispriming library specified
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq 
LEFT PRIMER         78   20   60.20   50.00  6.00  1.00 TTCCCATTCAGGAGCTCAAC
RIGHT PRIMER       570   21   59.40   47.62  4.00  3.00 GGTCTCTGAATATGGGCATTG
SEQUENCE SIZE: 623
INCLUDED REGION SIZE: 623

PRODUCT SIZE: 493, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

    1 GTCAAACAAATCTTCTTAGGGACAAGGTAGACACGTGTGCACTGAAACTCCCTGTCCTGT
                                                                  

   61 TGCCACCGAGCTTCCCTTTCCCATTCAGGAGCTCAACAGCATGTGAGAAGCCTCTTTGTG
                       >>>>>>>>>>>>>>>>>>>>                       

  121 AGGCCTTCAGGTGGTGTAGAAACTATTTCTATTTCTGAAAAGTTGCTTTTTTTATTGCTG
                                                                  

  181 GAGTCAGCCTCAAATATCTCTTAGGTCCATAGGCTTACCAGCTAAGGGGAAATTTAAAAT
                                                                  

  241 AAGTTATTTGGTTTGAAAAATAGTAGTATAGAAAATTGTAATGCAAGGCNNNNNNNNNNN
                                                                  

  301 NNNNNNNNNNNNNNNNNNNNNNNNNTAATTGGAGTAATGATCTTAAGTAGACAAGTACAT
                                                                  

  361 GTTTTACTCTTAAAATCAGTCAAATTGAGTATACATTGAGTAAATCAATTATCAGTTAAT
                                                                  

  421 TTGTTGGTAATTCAAATTGAAATTCCCACAGCTTATTCTTTACAAGAATGATACACTGAT
                                                                  

  481 CTCCCTCCTGTGAAGTCCTCTCTTGCTCCAACTTCTCATCTCATTATCACTCTGCAAAGG
                                                                  

  541 AAATATCATCAATGCCCATATTCAGAGACCACATTGATAGTGTCTTTTAGTTCAAAAATT
               <<<<<<<<<<<<<<<<<<<<<                              

  601 TAGATGTAGTTGTTAATTATGAA
                             

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq 

 1 LEFT PRIMER         32   20   60.36   55.00  6.00  0.00 CACGTGTGCACTGAAACTCC
   RIGHT PRIMER       510   21   59.59   52.38  3.00  3.00 TGGAGCAAGAGAGGACTTCAC
   PRODUCT SIZE: 479, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER         44   20   59.70   55.00  3.00  2.00 GAAACTCCCTGTCCTGTTGC
   RIGHT PRIMER       558   20   58.23   40.00  4.00  3.00 TGGGCATTGATGATATTTCC
   PRODUCT SIZE: 515, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER         80   20   60.79   55.00  6.00  1.00 CCCATTCAGGAGCTCAACAG
   RIGHT PRIMER       570   21   59.40   47.62  4.00  3.00 GGTCTCTGAATATGGGCATTG
   PRODUCT SIZE: 491, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER         69   20   60.57   50.00  4.00  1.00 AGCTTCCCTTTCCCATTCAG
   RIGHT PRIMER       558   20   58.23   40.00  4.00  3.00 TGGGCATTGATGATATTTCC
   PRODUCT SIZE: 490, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

Statistics
         con   too    in    in          no    tm    tm  high  high        high      
         sid  many   tar  excl   bad    GC   too   too   any    3'  poly   end      
        ered    Ns   get   reg   GC% clamp   low  high compl compl     X  stab    ok
Left    1746    55     0     0   147   266   702   293     0    37     8    26   212
Right   1730    55     0     0   204   281   831    99     0     8     6    15   231
Pair Stats:
considered 276, unacceptable product size 36, high any compl 18, high end compl 53, ok 169
primer3 release 0.9


(primer3_www_results.cgi v 0.1 beta 1a)