Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 78 20 60.20 50.00 6.00 1.00 TTCCCATTCAGGAGCTCAAC
RIGHT PRIMER 570 21 59.40 47.62 4.00 3.00 GGTCTCTGAATATGGGCATTG
SEQUENCE SIZE: 623
INCLUDED REGION SIZE: 623
PRODUCT SIZE: 493, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
1 GTCAAACAAATCTTCTTAGGGACAAGGTAGACACGTGTGCACTGAAACTCCCTGTCCTGT
61 TGCCACCGAGCTTCCCTTTCCCATTCAGGAGCTCAACAGCATGTGAGAAGCCTCTTTGTG
>>>>>>>>>>>>>>>>>>>>
121 AGGCCTTCAGGTGGTGTAGAAACTATTTCTATTTCTGAAAAGTTGCTTTTTTTATTGCTG
181 GAGTCAGCCTCAAATATCTCTTAGGTCCATAGGCTTACCAGCTAAGGGGAAATTTAAAAT
241 AAGTTATTTGGTTTGAAAAATAGTAGTATAGAAAATTGTAATGCAAGGCNNNNNNNNNNN
301 NNNNNNNNNNNNNNNNNNNNNNNNNTAATTGGAGTAATGATCTTAAGTAGACAAGTACAT
361 GTTTTACTCTTAAAATCAGTCAAATTGAGTATACATTGAGTAAATCAATTATCAGTTAAT
421 TTGTTGGTAATTCAAATTGAAATTCCCACAGCTTATTCTTTACAAGAATGATACACTGAT
481 CTCCCTCCTGTGAAGTCCTCTCTTGCTCCAACTTCTCATCTCATTATCACTCTGCAAAGG
541 AAATATCATCAATGCCCATATTCAGAGACCACATTGATAGTGTCTTTTAGTTCAAAAATT
<<<<<<<<<<<<<<<<<<<<<
601 TAGATGTAGTTGTTAATTATGAA
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 32 20 60.36 55.00 6.00 0.00 CACGTGTGCACTGAAACTCC
RIGHT PRIMER 510 21 59.59 52.38 3.00 3.00 TGGAGCAAGAGAGGACTTCAC
PRODUCT SIZE: 479, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
2 LEFT PRIMER 44 20 59.70 55.00 3.00 2.00 GAAACTCCCTGTCCTGTTGC
RIGHT PRIMER 558 20 58.23 40.00 4.00 3.00 TGGGCATTGATGATATTTCC
PRODUCT SIZE: 515, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
3 LEFT PRIMER 80 20 60.79 55.00 6.00 1.00 CCCATTCAGGAGCTCAACAG
RIGHT PRIMER 570 21 59.40 47.62 4.00 3.00 GGTCTCTGAATATGGGCATTG
PRODUCT SIZE: 491, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER 69 20 60.57 50.00 4.00 1.00 AGCTTCCCTTTCCCATTCAG
RIGHT PRIMER 558 20 58.23 40.00 4.00 3.00 TGGGCATTGATGATATTTCC
PRODUCT SIZE: 490, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 1746 55 0 0 147 266 702 293 0 37 8 26 212
Right 1730 55 0 0 204 281 831 99 0 8 6 15 231
Pair Stats:
considered 276, unacceptable product size 36, high any compl 18, high end compl 53, ok 169
primer3 release 0.9
(primer3_www_results.cgi v 0.1 beta 1a)