Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 27 21 59.25 47.62 6.00 2.00 CACAGGTCTGTTGTTGTTTGC
RIGHT PRIMER 177 19 58.86 52.63 5.00 0.00 CCATTTCATGGAGGTCTGG
SEQUENCE SIZE: 182
INCLUDED REGION SIZE: 182
PRODUCT SIZE: 151, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
1 TCCCGTTCCCCTGCCGCCCTTGCTCACACAGGTCTGTTGTTGTTTGCATAATCTGGGAGA
>>>>>>>>>>>>>>>>>>>>>
61 GGAAACCGAGCCCCTTTCGGCTGAGACCTCAGGCGCCAAAGCCTGCAGTTGGACAGATAG
121 TCCCAGCTGGTTTTCCGTTCCCTGGTCGCCCCCATGGGCCAGACCTCCATGAAATGGGGG
<<<<<<<<<<<<<<<<<<<
181 AG
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 27 21 59.25 47.62 6.00 2.00 CACAGGTCTGTTGTTGTTTGC
RIGHT PRIMER 178 19 58.86 52.63 5.00 1.00 CCCATTTCATGGAGGTCTG
PRODUCT SIZE: 152, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER 26 22 60.12 45.45 6.00 2.00 ACACAGGTCTGTTGTTGTTTGC
RIGHT PRIMER 177 19 58.86 52.63 5.00 0.00 CCATTTCATGGAGGTCTGG
PRODUCT SIZE: 152, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER 26 22 60.12 45.45 6.00 2.00 ACACAGGTCTGTTGTTGTTTGC
RIGHT PRIMER 178 19 58.86 52.63 5.00 1.00 CCCATTTCATGGAGGTCTG
PRODUCT SIZE: 153, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER 27 21 59.25 47.62 6.00 2.00 CACAGGTCTGTTGTTGTTTGC
RIGHT PRIMER 178 20 62.60 55.00 5.00 0.00 CCCATTTCATGGAGGTCTGG
PRODUCT SIZE: 152, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 422 0 0 0 1 41 43 277 0 11 0 9 40
Right 465 0 0 0 1 35 27 347 0 4 0 11 40
Pair Stats:
considered 140, unacceptable product size 97, high end compl 1, ok 42
primer3 release 0.9
(primer3_www_results.cgi v 0.1 beta 1a)