QC statistics for lane 7


Date Flowcell Lane Platform Unit Readgroup Sample Library Type Project Genome Centre
2016-09-02 HCVKHBBXX 7 160831_K00150_0116_BHCVKHBBXX_7 WTCHG_310912 9 samples 215/16_MPX_10nM Our indexes RNA-Seq PolyA P160524 GRCh37.EBVB95-8wt.ERCC WTCHG

Lane Length Tiles Clusters % PF Yield (Mrd) Yield (Mb) Yield (Mb Q20) % Mapped % Coverage % Primer % Broken % Variants Hets Mean cov.* % high cov. % dups % pair dups Link
7.1 75 88 4146507 100.0 364.89 27366.95 27118.11 88.8 5.4 0.00 2.9 2.36 ± 0.00 .0023 16.26 24.67 63.62 22.28 back
7.2 75 88 4146507 100.0 364.89 27366.95 26681.84 88.0 5.4 0.00 2.9 3.49 ± 0.15 .0020 15.85 24.41 57.78 21.21

  Fraction of reference that is covered at least once      Estimated heterozygosity (average over multiplexes). Sample contamination can increase this estimate. Filters: base quality >= 39, mapping quality >= 30, pairs properly mapped, no indels, maximum 2 high-quality (Q30) variants in read, insert size > read length.   *   Mean coverage is computed over regions that are covered at least once      Proportion of reads in regions with coverage in top 0.1 percentile   


Lane QC statistics and plots


Lane % GC % GCmapped σpos(%GC) insert ± MAD % exonic % exon cov'ge %N maxpos %N %lowQ %lowQend avgQ
7.1 48.9 ± 10.2 48.7 ± 10.4 4.70 171 ± 50 37.6 61.1 0.0 1.7 0.0 0.0 33.9
7.2 48.8 ± 10.6 48.7 ± 10.9 2.72 172 ± 47 39.4 62.4 0.1 2.2 0.1 0.1 31.6

G+C histogram
Insert size histogram
Mapped coverage by G+C

Coverage histogram
Exon/genome coverage distribution
Genomic coverage by G+C

(Predicted) variants by cycle (read 1)
Fraction N/lowQ, read 1
G+C by cycle (PF), read 1

Mean Q by cycle, read 1
Q score histogram, read 1
Variants by Q, read 1

(Predicted) variants by cycle (read 2)
Fraction N/lowQ, read 2
G+C by cycle (PF), read 2

Mean Q by cycle, read 2
Q score histogram, read 2
Variants by Q, read 2

Variants by GC
Indel rate by homopolymer content
Legend


  The Fraction N/Low Q plots, and dotted lines on the GC histogram plots, refer to all reads that have passed chastity filters. If a reference genome was available, all others refer to mapped reads, otherwise they too refer to chastity-filtered reads. The dotted lines in the fraction N/lowQ plot correspond to the fraction of bases with quality score 4 or less.      "Predicted variants" (dashed line) is the expected error frequency expressed as a Phred score, and may be compared with the "Variants by cycle" graph (solid line). "Mean Q" (solid) is the numerical mean Q score and is a measure of the average information content per read. These graphs use mapped reads only; the dashed line in the Mean Q plot uses all (PF) reads. All four graphs are calculated on called bases with Q Phred score above 4 only.      Mapped coverage by G+C. The coverage was averaged over those genomic regions that were covered at least once. Regions with coverage in the top 0.1 percentile were excluded; the dotted line shows results for all reads. The G+C fraction was computed from read bases, excluding Ns and bases with quality below 4.      Genomic coverage by G+C. The G+C fraction was computed from the reference genome, over the approximate fragment Regions with coverage in the top 0.1 percentile were excluded. The G+C histogram is shown as a dotted line (arbitrary Y scale).      The insert size distribution is summarized by the median and median absolute deviation.   




Multiplex QC statistics

Index Tag Readgroup Sample name Sample id Library Type Genome Project
201 TGTTAACT WTCHG_310912_201 HO3 CHA3768A1 Our indexes RNA-Seq PolyA GRCh37.EBVB95-8wt.ERCC P160524
202 TCGCGCGT WTCHG_310912_202 HB3 CHA3768A2 Our indexes RNA-Seq PolyA GRCh37.EBVB95-8wt.ERCC P160524
203 TAGAAGAG WTCHG_310912_203 MOTH CHA3768A3 Our indexes RNA-Seq PolyA GRCh37.EBVB95-8wt.ERCC P160524
204 GCTCTCTC WTCHG_310912_204 CD4COCO CHA3768A4 Our indexes RNA-Seq PolyA GRCh37.EBVB95-8wt.ERCC P160524
205 GAGTACTG WTCHG_310912_205 CDCOCB CHA3768A5 Our indexes RNA-Seq PolyA GRCh37.EBVB95-8wt.ERCC P160524
206 CTTGATTG WTCHG_310912_206 CD4MRO CHA3768A6 Our indexes RNA-Seq PolyA GRCh37.EBVB95-8wt.ERCC P160524
207 CTATATAC WTCHG_310912_207 CD4MRB CHA3768A7 Our indexes RNA-Seq PolyA GRCh37.EBVB95-8wt.ERCC P160524
208 CATTAATC WTCHG_310912_208 CD4THO CHA3768A8 Our indexes RNA-Seq PolyA GRCh37.EBVB95-8wt.ERCC P160524
209 TGTTGGTC WTCHG_310912_209 CD4THB CHA3768A9 Our indexes RNA-Seq PolyA GRCh37.EBVB95-8wt.ERCC P160524

Lane Mx Sample name Sample id Mean cvg Median cvg % > 10X % > 20X Exonic mean cvg Exonic median cvg Exonic % > 10X Exonic % > 20X
7 201 HO3 CHA3768A1 8.96 2 1.0 0.6 32.25 14 38.8 26.8
7 202 HB3 CHA3768A2 9.85 2 1.1 0.7 36.91 16 41.6 29.6
7 203 MOTH CHA3768A3 8.93 2 0.9 0.6 31.54 13 35.9 24.2
7 204 CD4COCO CHA3768A4 8.90 2 1.0 0.6 34.02 16 38.4 27.9
7 205 CDCOCB CHA3768A5 7.76 2 1.0 0.6 30.42 14 36.9 26.1
7 206 CD4MRO CHA3768A6 7.79 2 1.1 0.7 32.45 16 39.2 28.5
7 207 CD4MRB CHA3768A7 7.74 2 1.1 0.7 34.88 16 40.4 29.7
7 208 CD4THO CHA3768A8 8.62 2 1.0 0.7 35.96 17 39.8 29.4
7 209 CD4THB CHA3768A9 7.41 2 1.0 0.6 30.19 14 37.2 26.1

Lane Mx Yield Mrd Yield Mb Q20 % mapped % variants hets % exonic % exon cvg % pair dups most frequent kmer % m/f kmer % GC % GCmapped σpos (%GC) insert ± MAD %lowQ avg Q
7.1 All 364.89 27118.11 88.8 2.36 .0023 37.6 61.1 22.28 N/A 0.00 48.9 ± 10.2 48.7 ± 10.4 4.70 171 ± 50 0.0 33.9
7.1 201 41.30 3068.81 88.3 2.42 .0021 39.5 61.7 23.94 CACACACACA 0.03 49.1 ± 10.4 48.8 ± 10.6 4.67 177 ± 54 0.0 33.9
7.1 202 47.43 3523.99 87.8 2.60 .0023 41.5 63.4 27.65 GGCCAATTGA 0.03 49.9 ± 10.4 49.7 ± 10.6 4.63 170 ± 50 0.0 33.9
7.1 203 42.72 3175.09 88.0 2.39 .0021 39.5 59.9 23.63 GTCATTTTTG 0.07 50.1 ± 10.2 50.0 ± 10.4 4.62 169 ± 49 0.0 33.9
7.1 204 38.95 2896.06 89.0 2.38 .0023 37.1 60.1 21.97 TTTTTTTTTT 0.04 48.6 ± 10.1 48.5 ± 10.2 4.76 170 ± 49 0.0 34.0
7.1 205 36.20 2690.29 89.4 2.22 .0023 34.9 59.5 20.24 TTTTTTTTTT 0.05 47.8 ± 10.1 47.6 ± 10.2 4.78 174 ± 51 0.0 33.9
7.1 206 37.45 2782.92 89.3 2.22 .0023 36.1 61.4 18.61 TTTTTTTTTT 0.04 48.3 ± 10.2 48.1 ± 10.4 4.72 171 ± 48 0.0 33.9
7.1 207 43.33 3219.92 89.5 2.30 .0025 35.8 62.8 20.96 GGCCAATTGA 0.04 48.7 ± 10.2 48.6 ± 10.3 4.73 170 ± 47 0.0 33.9
7.1 208 40.29 2994.24 88.9 2.43 .0021 38.7 61.0 21.83 TTTTTTTTTT 0.06 48.9 ± 10.1 48.8 ± 10.2 4.70 167 ± 47 0.0 33.9
7.1 209 37.23 2766.79 89.8 2.21 .0023 34.4 60.1 19.98 TTTTTTTTTT 0.04 47.9 ± 10.2 47.8 ± 10.4 4.76 175 ± 52 0.0 33.9
7.2 All 364.89 26681.84 88.0 3.49 .0020 39.4 62.4 21.21 N/A 0.00 48.8 ± 10.6 48.7 ± 10.9 2.72 172 ± 47 0.1 31.6
7.2 201 41.30 3016.43 87.4 3.56 .0019 41.4 63.2 22.85 GCCAGAACAC 0.02 49.0 ± 11.0 48.7 ± 11.2 2.65 177 ± 51 0.1 31.5
7.2 202 47.43 3466.71 87.1 3.70 .0020 43.3 64.9 26.54 GCCAGAACAC 0.02 49.9 ± 10.8 49.6 ± 11.0 2.73 171 ± 47 0.1 31.6
7.2 203 42.72 3130.21 87.1 3.65 .0019 41.6 61.5 22.61 CGCGGGTCTG 0.10 50.2 ± 10.6 49.9 ± 10.9 2.75 171 ± 48 0.1 31.7
7.2 204 38.95 2857.56 88.2 3.42 .0021 39.0 61.4 20.88 CACATGCCTA 0.02 48.5 ± 10.5 48.4 ± 10.7 2.75 171 ± 46 0.1 31.9
7.2 205 36.20 2651.48 88.5 3.27 .0021 36.6 60.9 19.13 GTTGACTATT 0.02 47.6 ± 10.5 47.3 ± 10.6 2.70 175 ± 48 0.1 31.8
7.2 206 37.45 2733.52 88.5 3.39 .0021 37.6 62.5 17.60 TTTTTTTTTT 0.04 48.2 ± 10.6 47.9 ± 10.8 2.70 172 ± 46 0.1 31.5
7.2 207 43.33 3162.46 88.7 3.47 .0023 37.3 64.0 20.01 TTTTTTTTTT 0.05 48.6 ± 10.6 48.5 ± 10.8 2.72 171 ± 45 0.1 31.4
7.2 208 40.29 2945.92 88.0 3.54 .0019 40.4 62.3 20.67 TTTTTTTTTT 0.05 49.0 ± 10.4 48.9 ± 10.7 2.78 167 ± 45 0.1 31.6
7.2 209 37.23 2717.56 88.9 3.34 .0021 35.9 61.2 18.92 GTTGACTATT 0.02 47.8 ± 10.7 47.6 ± 10.9 2.67 176 ± 50 0.1 31.5

Yield (Mrd)
Median insert size
MAD insert size

Mean G+C
Std. dev. G+C by reads
Std. dev. G+C by position


Tile QC statistics and plots


Variant rate by tile (read 1)
Raw/mapped yield by tile (read 1)
Fraction N/lowQ by tile (read 1)

Variant rate by tile (read 2)
Raw/mapped yield by tile (read 2)
Fraction N/lowQ by tile (read 2)

  HiSeq tiles are grouped in order: swathe 1 top; swathe 1 bottom; swathe 2 top; etc.   


Lane Tile Yield (pf, Mrd) Variant rate (%) Mapped (%) Average Q N bases (%) LowQ (%) Last base lowQ (%)
7.1 1 3.373 2.11 88.89 39.58 0.01 0.01 0.02
7.1 2 3.404 2.11 88.94 39.64 0.01 0.01 0.02
7.1 3 3.444 2.12 88.93 39.65 0.01 0.01 0.02
7.1 4 3.391 2.11 88.92 39.64 0.01 0.01 0.02
7.1 5 3.388 2.10 88.96 39.64 0.01 0.01 0.02
7.1 6 3.339 2.11 88.94 39.57 0.01 0.01 0.02
7.1 7 3.358 2.10 88.93 39.59 0.01 0.01 0.02
7.1 8 3.330 2.11 88.94 39.59 0.01 0.01 0.02
7.1 9 3.356 2.11 88.92 39.62 0.01 0.01 0.02
7.1 10 3.380 2.11 88.94 39.60 0.01 0.01 0.02
7.1 11 3.356 2.11 88.89 39.57 0.01 0.01 0.02
7.1 12 3.373 2.10 88.93 39.60 0.01 0.01 0.02
7.1 13 3.320 2.11 88.90 39.56 0.01 0.01 0.02
7.1 14 3.377 2.10 88.90 39.62 0.01 0.01 0.02
7.1 15 3.362 2.12 88.90 39.56 0.01 0.01 0.02
7.1 16 3.366 2.12 88.92 39.62 0.01 0.01 0.02
7.1 17 3.390 2.11 88.95 39.62 0.01 0.01 0.02
7.1 18 3.410 2.11 88.94 39.64 0.01 0.01 0.02
7.1 19 3.399 2.11 88.95 39.64 0.01 0.01 0.02
7.1 20 3.351 2.11 88.94 39.62 0.01 0.01 0.02
7.1 21 6.503 2.09 88.87 39.57 0.02 0.02 0.04
7.1 22 6.559 2.09 88.88 39.60 0.03 0.03 0.04
7.1 23 6.536 2.10 88.87 39.59 0.03 0.03 0.04
7.1 24 6.563 2.10 88.86 39.60 0.03 0.03 0.04
7.1 25 6.556 2.09 88.87 39.60 0.03 0.03 0.04
7.1 26 6.505 2.10 88.87 39.57 0.03 0.03 0.04
7.1 27 6.499 2.27 88.66 39.55 0.03 0.03 0.04
7.1 28 6.558 2.09 88.78 39.60 0.03 0.03 0.04
7.1 29 3.152 2.09 88.74 39.50 0.05 0.05 0.07
7.1 30 3.175 2.08 88.70 39.53 0.05 0.05 0.07
7.1 31 3.192 2.08 88.74 39.54 0.05 0.05 0.07
7.1 32 3.181 2.08 88.71 39.53 0.05 0.05 0.07
7.1 33 3.193 2.08 88.73 39.56 0.05 0.05 0.07
7.1 34 3.184 2.08 88.69 39.55 0.05 0.05 0.07
7.1 35 3.185 2.09 88.72 39.55 0.05 0.05 0.07
7.1 36 3.198 2.08 88.74 39.55 0.05 0.05 0.07
7.1 37 3.182 2.07 88.72 39.52 0.05 0.05 0.07
7.1 38 3.208 2.08 88.74 39.55 0.05 0.05 0.07
7.1 39 3.186 2.08 88.73 39.53 0.05 0.05 0.07
7.1 40 3.134 2.09 88.74 39.49 0.05 0.05 0.07
7.1 41 6.475 2.09 88.82 39.52 0.02 0.02 0.04
7.1 42 6.493 2.10 88.85 39.56 0.02 0.02 0.04
7.1 43 6.506 2.10 88.82 39.54 0.02 0.02 0.03
7.1 44 6.489 2.09 88.84 39.54 0.02 0.02 0.03
7.1 45 6.491 2.09 88.84 39.56 0.03 0.03 0.04
7.1 46 6.476 2.09 88.83 39.54 0.03 0.03 0.04
7.1 47 6.487 2.10 88.87 39.56 0.03 0.03 0.04
7.1 48 6.508 2.09 88.86 39.58 0.03 0.03 0.04
7.1 49 3.379 2.10 88.96 39.64 0.01 0.01 0.01
7.1 50 3.391 2.10 88.95 39.66 0.01 0.01 0.01
7.1 51 3.395 2.10 88.95 39.64 0.01 0.01 0.01
7.1 52 3.371 2.10 88.95 39.58 0.01 0.01 0.01
7.1 53 3.387 2.10 88.95 39.63 0.01 0.01 0.01
7.1 54 3.384 2.10 88.93 39.63 0.01 0.01 0.01
7.1 55 3.364 2.10 88.97 39.62 0.01 0.01 0.01
7.1 56 3.406 2.11 88.96 39.64 0.01 0.01 0.01
7.1 57 3.410 2.10 88.99 39.65 0.01 0.01 0.01
7.1 58 3.396 2.10 88.96 39.63 0.01 0.01 0.01
7.1 59 3.394 2.10 88.96 39.62 0.01 0.01 0.01
7.1 60 3.360 2.11 88.97 39.57 0.01 0.01 0.01
7.1 61 6.413 2.09 88.87 39.51 0.02 0.02 0.03
7.1 62 6.457 2.09 88.91 39.54 0.02 0.02 0.03
7.1 63 6.470 2.10 88.85 39.53 0.02 0.02 0.03
7.1 64 6.481 2.09 88.88 39.54 0.02 0.02 0.03
7.1 65 6.461 2.09 88.86 39.54 0.02 0.02 0.03
7.1 66 6.456 2.10 88.89 39.52 0.02 0.02 0.03
7.1 67 6.482 2.10 88.87 39.55 0.02 0.02 0.03
7.1 68 6.524 2.09 88.87 39.56 0.02 0.02 0.03
7.1 69 3.120 2.08 88.73 39.46 0.04 0.04 0.06
7.1 70 3.115 2.08 88.73 39.47 0.04 0.04 0.06
7.1 71 3.139 2.09 88.71 39.48 0.04 0.04 0.06
7.1 72 3.148 2.08 88.78 39.50 0.04 0.04 0.06
7.1 73 3.167 2.08 88.74 39.53 0.04 0.04 0.06
7.1 74 3.176 2.08 88.71 39.53 0.05 0.05 0.06
7.1 75 3.162 2.08 88.73 39.50 0.05 0.05 0.06
7.1 76 3.172 2.08 88.72 39.53 0.05 0.05 0.06
7.1 77 3.166 2.08 88.71 39.51 0.05 0.05 0.05
7.1 78 3.158 2.08 88.73 39.49 0.04 0.04 0.05
7.1 79 3.146 2.08 88.70 39.47 0.04 0.04 0.05
7.1 80 3.139 2.08 88.71 39.47 0.04 0.04 0.05
7.1 81 3.112 2.09 88.75 39.45 0.03 0.03 0.05
7.1 82 3.144 2.09 88.78 39.49 0.03 0.03 0.04
7.1 83 3.137 2.08 88.77 39.49 0.03 0.03 0.04
7.1 84 3.127 2.08 88.81 39.46 0.03 0.03 0.04
7.1 85 3.102 2.08 88.78 39.46 0.04 0.04 0.05
7.1 86 3.088 2.08 88.77 39.44 0.04 0.04 0.05
7.1 87 3.074 2.09 88.78 39.44 0.04 0.04 0.05
7.1 88 3.081 2.08 88.79 39.43 0.04 0.04 0.05

Lane Tile Yield (pf, Mrd) Variant rate (%) Mapped (%) Average Q N bases (%) LowQ (%) Last base lowQ (%)
7.2 1 3.373 3.00 88.25 39.03 0.03 0.03 0.05
7.2 2 3.404 2.94 88.31 39.15 0.03 0.03 0.05
7.2 3 3.444 2.97 88.24 39.14 0.03 0.03 0.06
7.2 4 3.391 2.92 88.29 39.15 0.03 0.03 0.06
7.2 5 3.388 2.88 88.29 39.18 0.04 0.04 0.07
7.2 6 3.339 2.92 88.27 39.07 0.03 0.03 0.06
7.2 7 3.358 2.90 88.27 39.08 0.05 0.05 0.10
7.2 8 3.330 2.84 88.31 39.17 0.03 0.03 0.06
7.2 9 3.356 2.83 88.32 39.21 0.04 0.04 0.08
7.2 10 3.380 2.88 88.28 39.13 0.04 0.04 0.10
7.2 11 3.356 2.95 88.19 38.96 0.03 0.03 0.07
7.2 12 3.373 2.85 88.26 39.16 0.04 0.04 0.08
7.2 13 3.320 2.88 88.25 39.13 0.03 0.03 0.06
7.2 14 3.377 2.85 88.21 39.16 0.04 0.04 0.08
7.2 15 3.362 2.85 88.25 39.14 0.04 0.04 0.08
7.2 16 3.366 2.82 88.24 39.22 0.03 0.03 0.07
7.2 17 3.390 2.78 88.27 39.25 0.04 0.04 0.08
7.2 18 3.410 2.80 88.27 39.30 0.05 0.05 0.09
7.2 19 3.399 2.83 88.27 39.22 0.04 0.04 0.08
7.2 20 3.351 2.83 88.27 39.21 0.04 0.04 0.06
7.2 21 6.503 3.12 88.06 38.63 0.05 0.05 0.11
7.2 22 6.559 3.14 88.04 38.64 0.05 0.05 0.11
7.2 23 6.536 3.13 88.05 38.65 0.05 0.05 0.11
7.2 24 6.563 3.09 88.07 38.73 0.05 0.05 0.12
7.2 25 6.556 3.11 88.04 38.66 0.05 0.05 0.12
7.2 26 6.505 3.12 88.02 38.62 0.05 0.05 0.12
7.2 27 6.499 3.16 87.98 38.74 0.06 0.06 0.12
7.2 28 6.558 3.97 87.75 38.50 0.07 0.07 0.13
7.2 29 3.152 3.48 87.64 37.96 0.13 0.13 0.25
7.2 30 3.175 3.60 87.53 37.96 0.13 0.13 0.25
7.2 31 3.192 3.40 87.68 38.12 0.14 0.14 0.25
7.2 32 3.181 3.37 87.71 38.16 0.12 0.12 0.21
7.2 33 3.193 3.38 87.69 38.18 0.12 0.12 0.18
7.2 34 3.184 3.41 87.67 38.14 0.10 0.10 0.14
7.2 35 3.185 3.38 87.73 38.20 0.10 0.10 0.14
7.2 36 3.198 3.34 87.70 38.24 0.12 0.12 0.21
7.2 37 3.182 3.41 87.64 38.09 0.13 0.13 0.24
7.2 38 3.208 3.29 87.71 38.24 0.13 0.13 0.28
7.2 39 3.186 2.60 87.65 38.15 0.11 0.11 0.19
7.2 40 3.134 2.64 87.63 38.01 0.09 0.09 0.12
7.2 41 6.475 3.29 87.89 38.41 0.05 0.05 0.07
7.2 42 6.493 3.17 87.99 38.59 0.05 0.05 0.07
7.2 43 6.506 3.24 87.90 38.45 0.06 0.06 0.08
7.2 44 6.489 3.11 87.99 38.59 0.06 0.06 0.07
7.2 45 6.491 2.80 87.95 38.59 0.06 0.06 0.08
7.2 46 6.476 3.18 87.94 38.54 0.06 0.06 0.08
7.2 47 6.487 3.13 87.97 38.61 0.06 0.06 0.08
7.2 48 6.508 2.75 87.96 38.65 0.06 0.06 0.08
7.2 49 3.379 2.84 88.29 39.14 0.04 0.04 0.04
7.2 50 3.391 2.83 88.30 39.23 0.03 0.03 0.04
7.2 51 3.395 2.89 88.25 39.10 0.04 0.04 0.04
7.2 52 3.371 2.86 88.29 39.11 0.04 0.04 0.04
7.2 53 3.387 2.83 88.29 39.17 0.04 0.04 0.06
7.2 54 3.384 2.82 88.28 39.23 0.03 0.03 0.05
7.2 55 3.364 2.85 88.31 39.15 0.03 0.03 0.05
7.2 56 3.406 2.80 88.29 39.24 0.04 0.04 0.05
7.2 57 3.410 2.81 88.30 39.25 0.04 0.04 0.06
7.2 58 3.396 2.85 88.24 39.18 0.04 0.04 0.06
7.2 59 3.394 2.87 88.23 39.13 0.04 0.04 0.05
7.2 60 3.360 2.89 88.26 39.05 0.04 0.04 0.04
7.2 61 6.413 3.20 88.07 38.51 0.04 0.04 0.08
7.2 62 6.457 3.14 88.10 38.60 0.04 0.04 0.08
7.2 63 6.470 3.17 88.07 38.58 0.04 0.04 0.09
7.2 64 6.481 3.14 88.08 38.60 0.04 0.04 0.09
7.2 65 6.461 3.16 88.04 38.51 0.04 0.04 0.10
7.2 66 6.456 3.12 88.07 38.59 0.04 0.04 0.10
7.2 67 6.482 3.09 88.06 38.66 0.05 0.05 0.11
7.2 68 6.524 3.13 88.04 38.62 0.05 0.05 0.11
7.2 69 3.120 3.42 87.69 38.02 0.10 0.10 0.22
7.2 70 3.115 3.34 87.75 38.17 0.11 0.11 0.22
7.2 71 3.139 3.38 87.73 38.10 0.11 0.11 0.23
7.2 72 3.148 3.39 87.79 38.14 0.11 0.11 0.23
7.2 73 3.167 3.36 87.79 38.22 0.12 0.12 0.24
7.2 74 3.176 3.37 87.78 38.20 0.12 0.12 0.24
7.2 75 3.162 3.34 87.77 38.23 0.12 0.12 0.24
7.2 76 3.172 3.30 87.80 38.29 0.12 0.12 0.26
7.2 77 3.166 3.30 87.75 38.20 0.12 0.12 0.26
7.2 78 3.158 3.30 87.77 38.19 0.11 0.11 0.25
7.2 79 3.146 3.37 87.66 38.02 0.11 0.11 0.25
7.2 80 3.139 3.33 87.71 38.08 0.11 0.11 0.26
7.2 81 3.112 3.28 87.80 38.18 0.08 0.08 0.17
7.2 82 3.144 2.58 87.74 38.11 0.06 0.06 0.10
7.2 83 3.137 2.55 87.80 38.15 0.06 0.06 0.10
7.2 84 3.127 2.57 87.81 38.11 0.06 0.06 0.09
7.2 85 3.102 2.56 87.81 38.14 0.07 0.07 0.10
7.2 86 3.088 2.57 87.73 38.03 0.07 0.07 0.10
7.2 87 3.074 2.56 87.82 38.14 0.07 0.07 0.10
7.2 88 3.081 3.37 87.76 38.05 0.07 0.07 0.10


Component Version
RunMode HiSeq4000
ApplicationVersion 3.3.52
FPGAVersion 10.37.13
CPLDVersion 3.0.0
RTAVersion 2.7.3
BaseSpaceBrokerVersion 2.5.2.28
ChemistryVersion Illumina_Bruno Fluidics Controller_0_v2.0420
RecipeFragmentVersion 3.3.7
bclToFastq 2.17.1.14
startPipeline 2.2
FCdetails.pm 2.2
QCVersion 2.4